Anti-Glucocorticoid Receptor (Ab-2) Mouse mAb (BuGR2)

Analysis Note

Positive Controlmouse liver extract

Application

Frozen Sections (5 g/ml)Immunoblotting (5 g/ml)Immunocytochemistry (5 g/ml)Immunoprecipitation (see comments)Paraffin Sections (5 g/ml)

General description

Anti-Glucocorticoid Receptor (Ab-2), mouse monoclonal, clone BuGR2, recognizes ~94-97 kDa glucocorticoid receptor in mouse liver. It is validated for WB, ICC, IP & IHC (Frozen & paraffin sections).

Recognizes the ~94-97 kDa glucocorticoid receptor in mouse liver tissue.

Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with P3-AgX-653 myeloma cells (see application references). Recognizes the ~94-97 kDa glucocorticoid receptor.

Immunogen

partially purified, rat glucocorticoid receptor

Rat

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Does not cross react with androgen, progesterone, estrogen or mineralocorticoid receptors. Enzymatic digestion and immunoblot analysis has shown that this antibody reacts with the undigested 97 kDa glucocorticoid receptor, a 17 kDa DNA-binding trypsin fragment, and a 45 kDa steroid and DNA-binding chymotrypsin fragment. Immunocytochemical localization of the glucocorticoid receptor is observed in the cytoplasm and nucleus, even in the presence of hormone. This antibody has also been reported to work for immunoprecipitation. Antibody should be titrated for optimal results in individual systems.

Hussien, A., et al. 1997. Proc. Natl. Acad. Sci. USA94, 1521.Pratt, W.B. and Toft, D.O., 1997. Endocrine Rev.18 306.Schumacher, M., et al. 1996. Dev. Neurosci.18, 6.Toran-Allerand, C.D. 1996. Dev. Neurosci.18, 36.Weigel, N.L. 1996. Biochem J.319, 657.Jones, K.J. 1994. Ann. N.Y. Acad. Sci.743, 141.Arnold, A.P. 1990. in Hormones, Brain and Behavior in Vertebrates, ed. Balthazart, J. (Karger, Basel), 82.

Packaging

100 µg in Plastic ampoule

Physical form

Lyophilized from PBS, pH 7.2.

Reconstitution

Resuspend the lyophilized antibody with 0.1 ml sterile PBS, pH 7.2. Be careful to reconstitute the entire contents of the vial; during shipment and handling, portions of the lyophilized pellet may have become dislodged and may not be in the bottom of the vial. Following reconstitution, aliquot and freeze (-20°C). Avoid freeze/thaw cycles of solutions.

Warning

Toxicity: Standard Handling (A)